Inoki S, Osaki H, Furuya M
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1981;250(1-2):182-90.
In situ microfluorometry of whole kinetoplast DNA (K-DNA) and nuclear DNA (N-DNA) from a single trypomastigote cell of Trypanosoma gambiense (strain Wellcome) and Trypanosoma cruzi (strain Tulahuen) was attempted by using the microfluorometer combined with a photon counter. The results obtained were summarized as follows; 1. The K-DNA value of 2K 1N (2 kinetoplasts and 1 nucleus) T. gambiense was about twofold as much as that of 1K 1N on. 2. The N-DNA value of 2K 1N T. gambiense was a little less than two times as much as that of 1K 1N T. gambiense, showing that the N-DNA had already bee increased nearly twofold before the nuclear division was initiated. 3. The K-DNA value of 1K 1N T. cruzi was about fivefold as much as that of 1K 1N T. gambiense. The N-DNA value was contrarily lower than that of T. gambiense, being approximately 70% of the latter. 4. Percent K-DNA/N-DNA value was, in average, about 7 in either 1K 1N or 2K 1N T. gambiense and about 50 in T. cruzi. 5. Percent K-DNA/N-DNA value in individual trypomastigote cell was variable, showing that the increase of K-DNA and N-DNA was not synchronized at least in the two species of trypanosomes used here. 6. The fact that K-DNA and N-DNA values varied from cell to cell although they do not divide, suggested the possibility of DNA increase in the trypomastigote of T. cruzi.
尝试使用结合光子计数器的显微荧光计对来自冈比亚锥虫（威康株）和克氏锥虫（图拉亨株）单个锥鞭毛体细胞的完整动基体DNA（K-DNA）和核DNA（N-DNA）进行原位显微荧光测定。所得结果总结如下：1. 2K 1N（2个动基体和1个细胞核）的冈比亚锥虫的K-DNA值约为1K 1N的两倍。2. 2K 1N的冈比亚锥虫的N-DNA值略小于1K 1N的冈比亚锥虫的两倍，表明在核分裂开始前N-DNA已增加近两倍。3. 1K 1N的克氏锥虫的K-DNA值约为1K 1N的冈比亚锥虫的五倍。相反，N-DNA值低于冈比亚锥虫，约为后者的70%。4. 在1K 1N或2K 1N的冈比亚锥虫中，K-DNA/N-DNA值平均约为7，在克氏锥虫中约为50。5. 单个锥鞭毛体细胞中的K-DNA/N-DNA值是可变的，表明至少在此处使用的两种锥虫中，K-DNA和N-DNA的增加不同步。6. K-DNA和N-DNA值在细胞间变化但细胞不分裂这一事实，提示克氏锥虫锥鞭毛体中DNA增加的可能性。
