Inoki S, Osaki H, Furuya M
Zentralbl Bakteriol Orig A. 1979 Jul;244(2-3):327-30.
Using a spectrofluorometer with the Zeiss Universal Micro-Spectrophotometer 1 (UMSP 1), both nuclear and kinetoplast DNA (N-DNA and K-DNA) in the trypomastigote form of Trypanosoma gambiense (Strain Wellcome) were measured in situ without being extracted. As the fluorescent dye, ethidium bromide was preferably employed because there was a very marked increase in the ethidium fluorescence when the dye was intercalated between the base paires of the DNA helix. According to this method, it became possible to demonstrate the existence of double-stranded DNA in both nucleus and kinetoplast clearer than before.
使用蔡司通用微型分光光度计1（UMSP 1）的荧光分光光度计，对冈比亚锥虫（威康菌株）的锥鞭毛体形式的核DNA和动基体DNA（N-DNA和K-DNA）进行原位测量，无需提取。作为荧光染料，优选使用溴化乙锭，因为当该染料插入DNA螺旋的碱基对之间时，溴化乙锭荧光会有非常明显的增加。根据这种方法，比以前更清楚地证明了细胞核和动基体中双链DNA的存在。
