Daynes R A, Thomas M, Alvarez V L, Sandberg L B
Connect Tissue Res. 1977;5(2):75-82. doi: 10.3109/03008207709152233.
Immunization of rabbits with either porcine tropoelastin, lung alpha-elastin, or aortic alpha-elastin resulted in the production of antibodies against the respective antigens. The assay of antibody activity with a radioimmunoassay indicated a high degree of cross reactivity between these three soluble elastin preparations. Through the use of competitive protein binding experiments it was possible to detect antigen-specific differences between tropoelastin and the two alpha-elastins. No antigenic differences were observed between lung or aortic alpha-elastin by any of the assay procedures uded in this investigation. Absorption of the various antisera with either insolubilized tropoelastin, lung elastin or aortic elastin allowed the preparation of non-crossreactive antibodies and provided further evidence for the antigenic differences between tropoelastin and the soluble alpha-elastins. The implications of the findings, as they relate to our current understanding of the molecular and structural arrangement of elastin and the elastin precursor, are discussed.
用猪原弹性蛋白、肺α-弹性蛋白或主动脉α-弹性蛋白免疫兔子，可产生针对相应抗原的抗体。用放射免疫测定法检测抗体活性表明，这三种可溶性弹性蛋白制剂之间存在高度交叉反应性。通过竞争性蛋白质结合实验，能够检测到原弹性蛋白与两种α-弹性蛋白之间的抗原特异性差异。在本研究中使用的任何检测方法均未观察到肺或主动脉α-弹性蛋白之间存在抗原差异。用不溶性原弹性蛋白、肺弹性蛋白或主动脉弹性蛋白吸收各种抗血清，可制备非交叉反应性抗体，并进一步证明原弹性蛋白与可溶性α-弹性蛋白之间存在抗原差异。讨论了这些发现与我们目前对弹性蛋白和弹性蛋白前体的分子和结构排列的理解之间的关系。
