Truedsson L, Sjöholm A G, Laurell A B
Acta Pathol Microbiol Scand C. 1981 Jun;89(3):161-6. doi: 10.1111/j.1699-0463.1981.tb02680.x.
Two assays based on hemolysis in gel were assessed for screening complement (C) component deficiencies. In one assay sensitized sheep erythrocyte (EA) were incorporated in agarose gel containing Ca2+ and Mg2+, in the other guinea pig erythrocytes (GpE) were used in the presence of Mg2+and EGTA. With few exceptions, fresh samples from healthy individuals produced homogeneous areas of complete hemolysis in both assays. Clearly aberrant patterns were observed in approximately 4% of healthy blood donors. Sera from patients having complete deficiencies of Clq, C2 or C4 produced clear lysis of GpE only, whereas in sera lacking C3 or C8 lysis was grossly impaired in both assays. Properdin deficient serum produced very slight lysis of GpE but normal lysis of EA. Reconstitution of these C-deficient sera gave normal lysis. Together, the two assays supplement immunochemical C3 and C4 determinations for screening out C disorders.
评估了两种基于凝胶中溶血的检测方法用于筛查补体（C）成分缺陷。在一种检测方法中，将致敏绵羊红细胞（EA）加入含有Ca2+和Mg2+的琼脂糖凝胶中，在另一种检测方法中，在Mg2+和乙二醇双四乙酸（EGTA）存在的情况下使用豚鼠红细胞（GpE）。除了少数例外，来自健康个体的新鲜样本在两种检测方法中均产生均匀的完全溶血区域。在大约4%的健康献血者中观察到明显异常的模式。Clq、C2或C4完全缺乏的患者血清仅使GpE产生明显溶血，而在缺乏C3或C8的血清中，两种检测方法中的溶血均严重受损。备解素缺乏的血清使GpE产生非常轻微的溶血，但使EA产生正常溶血。这些补体缺乏血清的重建产生正常溶血现象。这两种检测方法共同补充了免疫化学C3和C4检测，用于筛查补体紊乱。
