Inoue K, Kurosumi K
Arch Histol Jpn. 1981 Mar;44(1):71-85. doi: 10.1679/aohc1950.44.71.
There are three possibilities concerning the mechanism of increase in number of gonadotrophs in the anterior pituitary after castration: one is the cell division by mitosis of matured gonadotrophs, another is the differentiation of immature cells, and the third is conversion of some other cell types into gonadotrophs. In order to determine the above possibilities, light and electron microscopic examinations using a combination of immunocytochemistry and autoradiography were performed. Normal and castrated adult male rats were used. Morphometry indicated that a highly increased population of gonadotrophs reacted with anti-LH beta serum after castration. Increased numbers of mitoses and elevated incorporation of 3H-thymidine into the nuclear chromatin were observed in both anti-LH beta reactive cells and other pituitary cells after castration. Electron microscopic immunocytochemical observations demonstrated the presence of immature cells with a few immunoreactive secretory granules in the castrated pituitary. It is considered that mitosis is a major factor for proliferation of gonadotrophs in the anterior pituitary after castration, but the possibility of differentiation from immature cells into gonadotrophs cannot be ruled out. The third possibility could not be proven in this study.
关于阉割后垂体前叶促性腺激素细胞数量增加的机制，存在三种可能性：一种是成熟的促性腺激素细胞通过有丝分裂进行细胞分裂，另一种是未成熟细胞的分化，第三种是其他一些细胞类型转化为促性腺激素细胞。为了确定上述可能性，采用免疫细胞化学和放射自显影相结合的方法进行了光镜和电镜检查。使用正常和阉割的成年雄性大鼠。形态计量学表明，阉割后大量促性腺激素细胞与抗促黄体生成素β血清发生反应。阉割后，在抗促黄体生成素β反应性细胞和其他垂体细胞中均观察到有丝分裂数量增加以及3H-胸腺嘧啶核苷掺入核染色质的量增加。电镜免疫细胞化学观察显示，阉割后的垂体中存在含有少量免疫反应性分泌颗粒的未成熟细胞。据认为，有丝分裂是阉割后垂体前叶促性腺激素细胞增殖的主要因素，但未成熟细胞分化为促性腺激素细胞的可能性也不能排除。在本研究中无法证实第三种可能性。
