Manch J N
Can J Microbiol. 1981 Nov;27(11):1231-3. doi: 10.1139/m81-190.
A previously unmapped pan gene was localized in an Escherichia coli K-12 strain using Hfr and F' matings and by transduction studies. This Pan- mutation was shown by supplementation studies to impart a block in pantoate biosynthesis. The pan gene cotransduced with rha, metB, and argE placing it at 87 min.
利用高频重组(Hfr)和F' 接合以及转导研究,在一株大肠杆菌K-12菌株中定位了一个先前未定位的泛酸基因。通过补充研究表明,这种泛酸突变在泛解酸生物合成中造成了阻断。泛酸基因与鼠李糖、甲硫氨酸B和精氨酸E共转导,将其定位在87分钟处。