Suzuki A, Karol R A, Kundu S K, Marcus D M
Int J Cancer. 1981 Sep 15;28(3):271-6. doi: 10.1002/ijc.2910280304.
The K562 cell line, which was established from a patient with chronic myeloid leukemia in blast crisis, was thought to be myeloid, but recent data indicate that it is an undifferentiated erythroid cell line. We have found that the glycosphingolipid content of these cells differs considerably from that of mature erythrocytes. Globotetraosylceramide, the most abundant glycolipid of mature red cells, was not detected in K562 cells, and neither was globotriaosylceramide. The predominant neutral glycolipids of K562 cells are monohexaosylceramides, which are a mixture of glucosyl- and galactosylceramides, and lactotriaosyl- and lactoneotetraosylceramides were also detected. Secondly, gangliosides which contain N-acetylgalactosamine were much more abundant than those containing N-acetylglucosamine in K562 cells, in contrast to erythrocytes. The most abundant ganglioside of K562 cells, GM2, is present in trace quantities in erythrocytes. A third major difference between these two cells lies in their relative proportions of neutral glycolipids and gangliosides. The molar ratio of neutral glycolipids/gangliosides is approximately 15:1 in erythrocytes and 1:1 in K562 cells. These striking differences between K562 cells and mature erythrocytes indicate that glycolipids may be useful cell surface markers of normal erythrocyte differentiation, and of erythroleukemias.
K562细胞系源自一名处于急变期的慢性髓性白血病患者，曾被认为是髓系细胞系，但最近的数据表明它是一种未分化的红系细胞系。我们发现这些细胞的糖鞘脂含量与成熟红细胞的糖鞘脂含量有很大差异。在K562细胞中未检测到成熟红细胞中最丰富的糖脂——球四糖神经酰胺，也未检测到球三糖神经酰胺。K562细胞的主要中性糖脂是单己糖神经酰胺，它是葡萄糖基神经酰胺和半乳糖基神经酰胺的混合物，同时还检测到了乳糖三糖神经酰胺和乳糖新四糖神经酰胺。其次，与红细胞相反，在K562细胞中，含有N - 乙酰半乳糖胺的神经节苷脂比含有N - 乙酰葡糖胺的神经节苷脂丰富得多。K562细胞中最丰富的神经节苷脂GM2在红细胞中仅微量存在。这两种细胞的第三个主要差异在于它们中性糖脂和神经节苷脂的相对比例。在红细胞中，中性糖脂/神经节苷脂的摩尔比约为15:1，而在K562细胞中为1:1。K562细胞与成熟红细胞之间的这些显著差异表明，糖脂可能是正常红细胞分化以及红白血病有用的细胞表面标志物。
