Narasimhan R, Murray R K
Biochem J. 1979 Apr 1;179(1):199-211. doi: 10.1042/bj1790199.
In order to help determine whether alterations of the profiles of glycosphingolipids occur consistently in human tumours, the neutral glycosphingolipids and gangliosides of nine lung tumours (one adenocarcinoma, four squamous cell, two mixed adeno-squamous cell, one large cell and one oat-cell carcinomata) were analysed. The control tissue consisted of adjacent lung; it contained neutral glycosphingolipids corresponding in properties to glucosyl-, lactosyl-, globotriaosyl- and globotetraosyl-ceramides. All of the tumours also contained these four neutral glycosphingolipids. However, in addition, five of the tumours (two of the squamous, the large cell and the two mixed adeno-squamous cell carcinomata) contained neutral glycosphingolipids corresponding in properties to lactotriaosyl- and neolactotetraosyl-ceramides; these same tumours also exhibited higher amounts of lactosylceramide than the other tumours analysed. Both of the two former neutral glycosphingolipids and very substantial amounts of the latter neutral glycosphingolipid were detected in pneumonic lung and in polymorphonuclear leucocytes; it thus appears possible that these particular compounds were derived from these latter cells rather than from the tumour cells. The ganglioside patterns of the tumours were almost equivalent in complexity to that exhibited by the control lung tissue. This study shows that the profiles of two major classes of glycosphingolipids (neutral glycosphingolipids and gangliosides) occurring in lung tumours are almost as complex as those of the parent tissue, a finding in contrast with the notably simplified patterns of these lipids found in many cancer cells grown in vitro. It also suggests that when lactotriaosyl- and neolactotetraosyl-ceramides and high amounts of lactosylceramide are detected in human tumours, the possibility must be considered that these compounds are derived from polymorphonuclear leucocytes.
为了帮助确定糖鞘脂谱的改变是否在人类肿瘤中持续发生，对9例肺肿瘤（1例腺癌、4例鳞状细胞癌、2例腺鳞混合细胞癌、1例大细胞癌和1例燕麦细胞癌）的中性糖鞘脂和神经节苷脂进行了分析。对照组织为相邻的肺组织；它含有性质上与葡糖基神经酰胺、乳糖基神经酰胺、球三糖基神经酰胺和球四糖基神经酰胺相对应的中性糖鞘脂。所有肿瘤也都含有这四种中性糖鞘脂。然而，此外，其中5例肿瘤（2例鳞状细胞癌、大细胞癌和2例腺鳞混合细胞癌）还含有性质上与乳糖三糖基神经酰胺和新乳糖四糖基神经酰胺相对应的中性糖鞘脂；与其他分析的肿瘤相比，这些相同的肿瘤还表现出更高含量的乳糖基神经酰胺。在肺炎肺组织和多形核白细胞中检测到了前两种中性糖鞘脂以及大量的后一种中性糖鞘脂；因此，似乎这些特定化合物可能来源于后一种细胞而非肿瘤细胞。肿瘤的神经节苷脂模式在复杂性上几乎与对照肺组织所呈现的模式相当。这项研究表明，肺肿瘤中出现的两类主要糖鞘脂（中性糖鞘脂和神经节苷脂）的谱几乎与母体组织的谱一样复杂，这一发现与在许多体外培养的癌细胞中发现的这些脂质明显简化的模式形成对比。它还表明，当在人类肿瘤中检测到乳糖三糖基神经酰胺、新乳糖四糖基神经酰胺和大量乳糖基神经酰胺时，必须考虑这些化合物来源于多形核白细胞的可能性。
