Rich A, Harris A K
J Cell Sci. 1981 Aug;50:1-7. doi: 10.1242/jcs.50.1.1.
Peritoneal macrophages were plated out on a series of artificial haptotactic substrata consisting of grid patterns of vacuum-evaporated palladium metal alternating with hydrophobic untreated polystyrene and with hydrophilic sulphonated polystyrene. By their active locomotion the macrophages accumulated preferentially onto the less hydrophilic of either pair of these alternative substrata. This order of substratum preference is precisely the opposite to that shown by fibroblastic cells. Macrophages were also found to accumulate preferentially onto roughened culture surfaces as opposed to smooth ones, which is again opposite to the behaviour of fibroblasts. These opposite substratum preferences shown by macrophages may reflect physical as well as functional differences in their surfaces, and could serve as assay criteria for macrophages and cell types putatively equivalent to macrophages.
将腹膜巨噬细胞接种在一系列人工趋化性基质上，这些基质由真空蒸发钯金属的网格图案组成，交替排列着疏水的未处理聚苯乙烯和亲水的磺化聚苯乙烯。通过其活跃的移动，巨噬细胞优先聚集在这两种交替基质中亲水性较差的那种上。这种基质偏好顺序与成纤维细胞所显示的顺序正好相反。还发现巨噬细胞优先聚集在粗糙的培养表面上，而不是光滑的表面上，这同样与成纤维细胞的行为相反。巨噬细胞所显示的这些相反的基质偏好可能反映了其表面的物理和功能差异，并且可以作为巨噬细胞和假定等同于巨噬细胞的细胞类型的检测标准。
