Division of Dentistry, Medicine and Health Science, University of Melbourne, Melbourne, VIC, Australia.
Department of Periodontology, Indiana University School of Dentistry, Indianapolis, IN, United States.
Front Immunol. 2021 Jun 2;12:618002. doi: 10.3389/fimmu.2021.618002. eCollection 2021.
Cellular responses to implanted biomaterials are key to understanding osseointegration. The aim of this investigation was to determine the priming and activation of the respiratory burst activity of monocytes in response to surface-modified titanium. Human peripheral blood monocytes of healthy blood donors were separated, then incubated with surface-modified grade 2 commercially pure titanium (CPT) disks with a range of known surface energies and surface roughness for 30- or 60-min. Secondary stimulation by phorbol 12-myrisate 13-acetate (PMA) following the priming phase, and luminol-enhanced-chemiluminescence (LCL) was used to monitor oxygen-dependent activity. Comparison among groups was made by incubation time using one-way ANOVA. One sample from each group for each phase of the experiment was viewed under scanning electron microscopy (SEM) and qualitative comparisons made. The results indicate that titanium is capable of priming peripheral blood monocytes following 60-min incubation. In contrast, 30 min incubation time lead to reduced LCL on secondary stimulation as compared to cells alone. At both time intervals, the disk with the lowest surface energy produced significantly less LCL compared to other samples. SEM examination revealed differences in surface morphology at different time points but not between differently surface-modified disks. These results are consistent with the hypothesis that the titanium surface characteristics influenced the monocyte activity, which may be important in regulating the healing response to these materials.
细胞对植入生物材料的反应是理解骨整合的关键。本研究旨在确定表面改性钛对单核细胞呼吸爆发活性的预刺激和激活。从健康献血者中分离出人外周血单核细胞，然后用一系列已知表面能和表面粗糙度的商用纯钛（CPT）盘在 30 或 60 分钟孵育。在预刺激阶段后用佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）进行二次刺激，并使用发光增强化学发光（LCL）监测氧依赖性活性。通过单向方差分析比较组间差异。在实验的每个阶段，从每个组中取出一个样本进行扫描电子显微镜（SEM）观察，并进行定性比较。结果表明，钛在 60 分钟孵育后能够对外周血单核细胞进行预刺激。相比之下，30 分钟孵育时间导致二次刺激时的 LCL 减少，而单独的细胞则没有。在两个时间间隔内，表面能最低的圆盘产生的 LCL 明显少于其他样本。SEM 检查显示在不同时间点表面形态存在差异，但不同表面改性圆盘之间没有差异。这些结果与钛表面特性影响单核细胞活性的假设一致，这可能对调节对这些材料的愈合反应很重要。
