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奇异变形杆菌群体游动期间代谢活性的变化

Changes in metabolic activity of Proteus mirabilis during swarming.

作者信息

Armitage J P

出版信息

J Gen Microbiol. 1981 Aug;125(2):445-50. doi: 10.1099/00221287-125-2-445.

DOI:10.1099/00221287-125-2-445
PMID:7033472
Abstract

Proteus mirabilis in the long, swarming form had altered metabolic activity compared with bacteria in non-swarming phases on solid media. During swarming the rates of incorporation of precursors into DNA, RNA and protein, as measured in broth cultures immediately after harvesting from swarm plates, were lowered. The rates of uptake of these precursors into the bacteria were also lowered, and at the same time the rate of oxygen uptake was reduced to less than 20% of the normal rate, although intracellular ATP concentrations remained constant. The return of macromolecular synthesis and oxygen uptake to preswarming rates corresponded to the end of the active swarm period. The results indicate that in the multiflagellate swarmers of P. mirabilis metabolic activity was lowered to a level necessary to maintain flagella activity but not bacterial growth.

摘要

与固体培养基上非群体运动阶段的细菌相比,呈长链群体运动形态的奇异变形杆菌代谢活性发生了改变。在群体运动期间,从群体运动平板收获后立即在肉汤培养物中测量的前体掺入DNA、RNA和蛋白质的速率降低。这些前体进入细菌的摄取速率也降低,同时氧气摄取速率降至正常速率的20%以下,尽管细胞内ATP浓度保持恒定。大分子合成和氧气摄取恢复到群体运动前的速率与活跃群体运动期的结束相对应。结果表明,在奇异变形杆菌的多鞭毛群体运动菌中,代谢活性降低到维持鞭毛活性但不支持细菌生长所需的水平。

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