Moczar M
Pathol Biol (Paris). 1981 Nov;29(9):567-71.
Control and chloroquine treated fibroblast cultures were incubated with 14C-glucosamine. The cells were separated from the medium and the pericellular matrix. The S-carboxymethylproteins in the medium were hydrolysed with trypsin and pronase. The glycosaminoglycans were treated with hyaluronidase, chondroitin AB-lyase and chondroitin ABC-lyase. The macromolecular 14C label per cell in the medium was higher in the chloroquine treated cultures than in the controls. The incorporation of the 14C-hexosamines per cell into the pericellular and intracellular compartment was not modified. The increased 14C-label in the medium of fibroblast exposed to chloroquine accounted for the increased incorporation of the 14C precursor into chondroitin 4 and 6 sulfates and dermatan sulfate.
将对照和成氯喹处理的成纤维细胞培养物与¹⁴C - 葡糖胺一起孵育。将细胞与培养基和细胞周围基质分离。培养基中的S - 羧甲基蛋白用胰蛋白酶和链霉蛋白酶水解。糖胺聚糖用透明质酸酶、软骨素AB - 裂解酶和软骨素ABC - 裂解酶处理。与对照相比，氯喹处理的培养物中培养基中每个细胞的大分子¹⁴C标记更高。每个细胞中¹⁴C - 己糖胺掺入细胞周围和细胞内区室的情况未发生改变。暴露于氯喹的成纤维细胞培养基中¹⁴C标记的增加是由于¹⁴C前体掺入硫酸软骨素4和6以及硫酸皮肤素的增加。
