Milan L, Trachtenberg M C
Am J Surg Pathol. 1981 Sep;5(6):573-9. doi: 10.1097/00000478-198109000-00006.
Decalcification of bone specimens of 2-5 mm thickness can be achieved in 5 hours or less when the decalcifying fluids are agitated by ultrasonic energization. The morphology of hard and soft tissue is extremely well preserved by this procedure. Acid or chelating decalcifiers may be used and the application of combined fixation-chelation permits routinely many histochemical procedures previously requiring special handling.
当通过超声能量搅动脱钙液时,厚度为2 - 5毫米的骨标本可在5小时或更短时间内完成脱钙。通过该程序,硬组织和软组织的形态能得到极其良好的保存。可使用酸性或螯合性脱钙剂,联合固定 - 螯合的应用使得许多以前需要特殊处理的组织化学程序能够常规进行。