Schaffer R, Sniegoski L T, Welch M J, White V E, Cohen A, Hertz H S, Mandel J, Paule R C, Svensson L, Björkhem I, Blomstrand R
Clin Chem. 1982 Jan;28(1):5-8.
Isotope dilution/mass spectrometric methods for total serum cholesterol, developed separately at the Karolinska Institutet (KI) and the National Bureau of Standards (NBS), were compared by applying them to a common set of serum pools. A search for the cause of a systematic difference of a few percent in results from the two methods revealed that the KI cholesterol standard contained lathosterol, which interfered with the calibration of the method. With NBS Standard Reference Material cholesterol used for new analyses at the KI, the average difference in mean values dropped to 0.2%. The NBS results are more precise. This is attributed to the protocols NBS used for sample preparation and mass spectrometry. However, these protocols make the NBS method more complex and time-consuming. A recent critical article on the use of this technique for total cholesterol is also examined.
分别由卡罗林斯卡学院（KI）和国家标准局（NBS）开发的用于测定血清总胆固醇的同位素稀释/质谱法，通过将它们应用于一组共同的血清库进行了比较。对两种方法结果中存在百分之几的系统差异的原因进行探究后发现，KI胆固醇标准品中含有羊毛甾醇，这干扰了该方法的校准。在KI使用NBS标准参考物质胆固醇进行新的分析后，平均值的平均差异降至0.2%。NBS的结果更精确。这归因于NBS用于样品制备和质谱分析的方案。然而，这些方案使NBS方法更加复杂且耗时。还对最近一篇关于使用该技术测定总胆固醇的批判性文章进行了研究。
