Price M G, Lazarides E
J Cell Biol. 1983 Dec;97(6):1860-74. doi: 10.1083/jcb.97.6.1860.
The expression of two intermediate filament-associated proteins, paranemin (280,000 mol wt) and synemin (230,000 mol wt), was investigated with respect to the expression of two core intermediate filament proteins, desmin and vimentin, in various embryonic and adult chicken muscle and nonmuscle cells. All developing muscle cells, regardless of their type, simultaneously express desmin, vimentin, paranemin, and synemin. However, a difference is observed in the expression of paranemin in adult muscle. This protein is removed during differentiation of both fast and slow skeletal muscle, visceral smooth muscle, and the smooth muscle of muscular arteries, but remains in mature myocardial cells, cardiac conducting fibers, and the smooth muscle cells of elastic arteries. Some of these cells express vimentin, others desmin, and still others a mixture of the two. On the other hand, synemin is expressed in all the above types of adult muscle cells except myocardial cells. Adult myocardial cells also lack vimentin, and its presence is gradually reduced after hatching. Since in adult striated muscle all expressed intermediate filament proteins are found predominantly in association with the peripheries of myofibrillar Z discs, these results suggest that a change in the composition of skeletal and cardiac muscle Z discs occurs during chicken development and maturation. Erythrocytes that express synemin and vimentin do not express paranemin, while both embryonic and adult Schwann cells co-express paranemin and vimentin, but not synemin. Endothelial cells of muscular vessels express paranemin, while those of elastic vessels do not, and neither contains synemin. Paranemin and synemin are not expressed in neurons, epithelial, and most glial cells, suggesting that these two polypeptides are expressed only in conjunction with desmin or vimentin. These results suggest that the composition of intermediate filaments changes during chicken development, not only with respect to their core subunit proteins but also with respect to two associated polypeptides, particularly in muscle cells.
我们研究了两种中间丝相关蛋白，副肌动蛋白（分子量280,000）和伴肌动蛋白（分子量230,000），在各种胚胎和成年鸡肌肉及非肌肉细胞中，与两种核心中间丝蛋白，结蛋白和波形蛋白表达的相关性。所有发育中的肌肉细胞，无论其类型如何，均同时表达结蛋白、波形蛋白、副肌动蛋白和伴肌动蛋白。然而，在成年肌肉中副肌动蛋白的表达存在差异。在快、慢骨骼肌、内脏平滑肌和肌性动脉平滑肌的分化过程中，这种蛋白会被去除，但在成熟的心肌细胞、心脏传导纤维和弹性动脉的平滑肌细胞中保留。这些细胞中有些表达波形蛋白，有些表达结蛋白，还有些同时表达两者。另一方面，伴肌动蛋白在除心肌细胞外的上述所有类型成年肌肉细胞中表达。成年心肌细胞也缺乏波形蛋白，且其表达在孵化后逐渐减少。由于在成年横纹肌中，所有表达的中间丝蛋白主要与肌原纤维Z盘的周边相关，这些结果表明，在鸡的发育和成熟过程中，骨骼肌和心肌Z盘的组成发生了变化。表达伴肌动蛋白和波形蛋白的红细胞不表达副肌动蛋白，而胚胎和成年施万细胞同时表达副肌动蛋白和波形蛋白，但不表达伴肌动蛋白。肌性血管的内皮细胞表达副肌动蛋白，而弹性血管的内皮细胞不表达，且两者均不含有伴肌动蛋白。副肌动蛋白和伴肌动蛋白在神经元、上皮细胞和大多数神经胶质细胞中不表达，这表明这两种多肽仅与结蛋白或波形蛋白共同表达。这些结果表明，在鸡的发育过程中，中间丝的组成不仅在其核心亚基蛋白方面发生变化，而且在两种相关多肽方面也发生变化，尤其是在肌肉细胞中。
