Kearney J F, Barletta R, Quan Z S, Quintáns J
Eur J Immunol. 1981 Nov;11(11):877-83. doi: 10.1002/eji.1830111106.
Biological activities of monoclonal A/J antibodies to the T15 idiotype in BALB/c mice were compared to heterogeneous antibodies raised by conventional immunization procedures. Two monoclonal antibodies, AB1-2 and GB4-10, which are of the gamma 1, chi class, appeared to have identical specificities by binding criteria and reacted similarly to conventional antibodies in their abilities to induce neonatal suppression, inhibit plaque-forming cell induction by phosphorylcholine (PC) antigens and to inhibit specifically, anti-PC plaque-forming cells. However, in functional analyses of anti-PC responses in various strains of mice, discrepancies were noted in the T15 responses as defined by monoclonal antibodies and conventional antisera. This heterogeneity was also observed in adult mice suppressed with the GB4-10 monoclonal antibody. These animals eventually produced an anti-PC responses of AB1-2 idiotype but lacking the GB4-10 marker. These results show that the T15 IgM anti-PC response in BALB/c and other strains of mice is heterogeneous and probably consists of a family of clones. Particular clones can be precisely eliminated by the use of appropriate monoclonal antibodies, and the anti-PC response that eventually recovers is still T15+ but lacking the suppressed clones.
将BALB/c小鼠中针对T15独特型的单克隆A/J抗体的生物学活性与通过传统免疫程序产生的异种抗体进行了比较。两种γ1、κ类单克隆抗体AB1-2和GB4-10，根据结合标准似乎具有相同的特异性，并且在诱导新生抑制、抑制磷酸胆碱（PC）抗原诱导的噬斑形成细胞以及特异性抑制抗PC噬斑形成细胞的能力方面，与传统抗体的反应相似。然而，在对各种小鼠品系中抗PC反应的功能分析中，单克隆抗体和传统抗血清所定义的T15反应存在差异。在用GB4-10单克隆抗体抑制的成年小鼠中也观察到了这种异质性。这些动物最终产生了AB1-2独特型的抗PC反应，但缺乏GB4-10标记。这些结果表明，BALB/c和其他小鼠品系中的T15 IgM抗PC反应是异质性的，可能由一组克隆组成。通过使用适当的单克隆抗体可以精确消除特定的克隆，最终恢复的抗PC反应仍然是T15+，但缺乏被抑制的克隆。
