Wittner M K, Bach M A, Köhler H
J Immunol. 1982 Feb;128(2):595-9.
Hybridoma antibodies against the PC-binding T15 BALB/c myeloma protein were raised by cell fusion with anti-T15 A/He immune cells. The idiotype specificity of these monoclonal anti-T15 antibodies was determined with a panel of different myeloma and hybridoma immunoglobulins. Two types of anti-T15 antibodies are seen. One reacts with a number of different IgA myeloma proteins and with serum IgA of certain strains of mice; this reactivity most likely is due to allotypy. The other group consists of anti-T15 antibodies that are specific for the T15 idiotype and are therefore termed anti-idiotypic. The bindings of the anti-idiotype antibodies to T15 were specifically inhibited by T15 (F(ab')2 but not by other PC-binding myeloma proteins of different idiotypes. The relationship of the idiotype-specific anti-T15 antibodies to the PC-binding site of the T15 idiotype was analyzed by hapten inhibition of anti-idiotypic binding and by inhibition of BALB/c anti-PC splenic hemolytic plaque formation. Anti-T15 antibodies, for which the T15 binding is inhibited by PC or PC-BSA, also specifically inhibit anti-PC plaque formation. These antibodies are labeled site and near-site anti-idiotypic antibodies. Site and near-site-specific anti-idiotypic antibodies recognize different idiotopes on the T15 molecules. The possible differential biologic activities of these anti-idiotopes in idiotype network regulation is considered.
通过与抗 T15 A/He 免疫细胞进行细胞融合，制备了针对结合 PC 的 T15 BALB/c 骨髓瘤蛋白的杂交瘤抗体。利用一组不同的骨髓瘤和杂交瘤免疫球蛋白确定了这些单克隆抗 T15 抗体的独特型特异性。观察到两种类型的抗 T15 抗体。一种与多种不同的 IgA 骨髓瘤蛋白以及某些小鼠品系的血清 IgA 发生反应；这种反应性很可能是由于同种异型。另一组由对 T15 独特型具有特异性的抗 T15 抗体组成，因此被称为抗独特型。抗独特型抗体与 T15 的结合被 T15（F(ab')2）特异性抑制，但不被其他不同独特型的结合 PC 的骨髓瘤蛋白抑制。通过半抗原抑制抗独特型结合以及抑制 BALB/c 抗 PC 脾溶血空斑形成，分析了独特型特异性抗 T15 抗体与 T15 独特型的 PC 结合位点的关系。其 T15 结合被 PC 或 PC-BSA 抑制的抗 T15 抗体也特异性抑制抗 PC 空斑形成。这些抗体是位点和近位点抗独特型抗体。位点和近位点特异性抗独特型抗体识别 T15 分子上不同的独特位。考虑了这些抗独特位在独特型网络调节中可能的不同生物学活性。
