原始红细胞成熟系列上鸡胚胎抗原（CFA）的表达

Chicken fetal antigen (CFA) expression on the primitive erythroid maturation series.

作者信息

Patel N H, Nelson C H, Ellison J R, Sanders B G

出版信息

Exp Hematol. 1981 Sep;9(8):878-85.

Abstract

Indirect immunofluorescence was utilized to study the expression of chicken fetal antigens (CFA), developmentally phasic membrane antigens, on the primitive erythroid series during chick embryo development. Cells of this series expressed CFA determinants at all four stages of cellular maturation. However, the relative intensity of fluorescent staining (measurement of CFA determinants) varied with development. CFA determinant loss was correlated with the age of the embryo, and the stage of cellular maturation. Indirect immunofluorescence utilizing antisera capable of detecting eight individual CFA determinants revealed that all eight CFA determinants were present on early polychromatic erythrocytes of the primitive series, but that a selective and sequential loss of CFA determinants occurred in later stages. The data suggest that certain CFA determinants are differentially expressed during primitive erythroid cellular maturation in the developing chick embryo.

摘要

利用间接免疫荧光法研究鸡胚胎发育过程中原始红细胞系上鸡胎儿抗原（CFA）即发育阶段特异性膜抗原的表达。该细胞系的细胞在细胞成熟的所有四个阶段均表达CFA决定簇。然而，荧光染色的相对强度（CFA决定簇的测量值）随发育而变化。CFA决定簇的丢失与胚胎年龄和细胞成熟阶段相关。利用能够检测八个单独CFA决定簇的抗血清进行间接免疫荧光分析显示，原始红细胞系的早期多色红细胞上存在所有八个CFA决定簇，但在后期会发生CFA决定簇的选择性和顺序性丢失。数据表明，在发育中的鸡胚胎原始红细胞成熟过程中，某些CFA决定簇存在差异表达。