Bosman F T, Go P M
Histochemistry. 1981;73(2):195-9. doi: 10.1007/BF00493019.
Several methods for tissue embedding in polyethylene glycol (PEG) were compared with regard to their applicability for pre-embedding immunoelectronmicroscopy. Existing embedding procedures gave unsatisfactory results and therefore a modified procedure was developed. This method, consisting of very brief tissue infiltration with PEG 1500, to which 3% water is added, allowed adequate tissue sectioning. Using these sections for preembedding immunoelectronmicroscopical localisation of glucagon in bovine pancreatic islets adequate ultrastructural morphology was obtained in combination with excellent preservation of peptide hormone immunoreactivity.
就聚乙二醇（PEG）组织包埋方法在包埋前免疫电子显微镜检查中的适用性进行了几种方法的比较。现有的包埋程序结果不尽人意，因此开发了一种改进程序。该方法包括用添加了3%水的PEG 1500对组织进行极短时间的浸润，这样能进行充分的组织切片。使用这些切片对牛胰岛中的胰高血糖素进行包埋前免疫电子显微镜定位，在获得足够超微结构形态的同时，肽类激素免疫反应性得到了极佳的保存。
