Wong F S, Visintin I, Shanabrough M, Leranth C, Janeway C A
Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut, USA.
J Histochem Cytochem. 1998 Dec;46(12):1341-6. doi: 10.1177/002215549804601201.
We developed a simple method employing the use of flat-embedding techniques on thick frozen sections which allows correlation of light and electron microscopic immunohistochemistry. This method has been particularly useful in visualization of pancreas sections, an adaptation especially important because this tissue is not amenable to conventional vibratome sectioning. In this study we demonstrate the use of this technique to examine the same tissue section at the light and the electron microscopic level while maintaining morphology.
我们开发了一种简单的方法，该方法在厚冰冻切片上采用平埋技术，可实现光镜和电镜免疫组织化学的关联。这种方法在胰腺切片的可视化方面特别有用，这种适应性尤其重要，因为这种组织不适合传统的振动切片机切片。在本研究中，我们展示了使用该技术在保持形态的同时，在光镜和电镜水平上检查同一组织切片。
