Autilio-Gambetti L, Sipple J, Sudilovsky O, Gambetti P
J Neurochem. 1982 Mar;38(3):774-80. doi: 10.1111/j.1471-4159.1982.tb08698.x.
Intermediate filaments were prepared from distal stumps of rabbit sciatic nerve 5 weeks after nerve section, at which time Schwann cells account for 85--90% of the cell area. A polypeptide of molecular weight 58,000 was the main component of this fraction. An antiserum raised in guinea pig against this polypeptide stained all cells present in the distal stump, as well as Schwann cells and 3T3 cells in culture. The identity of the molecular weight 58,000 polypeptide obtained from distal stumps with vimentin was proved with one and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and with immunoautoradiography. It is concluded that the intermediate filament subunit of undifferentiated Schwann cells is vimentin. The possibility that Schwann cells in normal nerve may have another type of intermediate filament besides vimentin cannot be ruled out.
在兔坐骨神经切断后5周，从远端残端制备中间丝，此时雪旺细胞占细胞面积的85%-90%。分子量为58,000的一种多肽是该组分的主要成分。用豚鼠制备的针对该多肽的抗血清可使远端残端中的所有细胞以及培养中的雪旺细胞和3T3细胞染色。通过一维和二维十二烷基硫酸钠聚丙烯酰胺凝胶电泳以及免疫放射自显影，证实了从远端残端获得的分子量为58,000的多肽与波形蛋白相同。得出结论：未分化雪旺细胞的中间丝亚基是波形蛋白。不能排除正常神经中的雪旺细胞除波形蛋白外可能还有另一种中间丝的可能性。
