Beezer A E, Sharma P B
Microbios. 1981;31(124):71-82.
The electrophoretic behaviour of fresh and liquid nitrogen stored inocula of Saccharomyces cerevisiae has been studied as a function of pH, ionic strength, buffer composition, growth temperature of the inoculum, number of washings of the inoculum prior to electrophoretic investigation, growth medium used in the preparation of the inoculum, interaction of the inoculum with nystatin, and interaction of the inoculum with calcium ions. The results provide a basis for a discussion of the role of the yeast cell surface in the mode of action of nystatin (a polyene antibiotic), on interaction with sensitive Saccharomyces cerevisiae cells. They indicate the profound importance of the cell surface in the interaction with the antibiotic. Moreover, the results of the temperature growth/electrophoresis experiments support the view that a sharp change in membrane fluidity occurs in the yeast membrane. The results are also discussed in the light of those reported in previous papers on the interaction of yeast cells with nystatin.
研究了新鲜的和经液氮保存的酿酒酵母接种物的电泳行为，该行为是pH、离子强度、缓冲液组成、接种物生长温度、电泳研究前接种物洗涤次数、制备接种物所用生长培养基、接种物与制霉菌素的相互作用以及接种物与钙离子的相互作用的函数。这些结果为讨论酵母细胞表面在制霉菌素（一种多烯抗生素）作用方式中对与敏感酿酒酵母细胞相互作用的作用提供了基础。它们表明细胞表面在与抗生素相互作用中具有极其重要的意义。此外，温度生长/电泳实验结果支持了酵母细胞膜中膜流动性发生急剧变化的观点。还根据先前关于酵母细胞与制霉菌素相互作用的论文中报道的结果对这些结果进行了讨论。
