Zyrnowa J G, Członkowska A
Neurol Neurochir Pol. 1981 Jul-Aug;15(4):393-6.
Antibodies to brain tissue were determined by indirect immunofluorescence of Coons in 23 patients with hepatolenticular degeneration. In 14 patients the levels of immunoglobulins A, G and M were determined as well. Control investigations were done in 37 donors. Positive results of Coons reaction with bright fluorescence of nervous tissue antigens were obtained in patients with increased serum immunoglobulin levels. Qualitative differences were obtained also in the reaction of Coons with sera from various patients. These data evidence that immunoglobulin F (ab)2 fragments as well as Fc fragments participate in the immunofluorescent reaction which calls for caution in the interpretation of the results of this method.
采用库恩斯间接免疫荧光法检测了23例肝豆状核变性患者的脑组织抗体。对其中14例患者还测定了免疫球蛋白A、G和M的水平。对37名献血者进行了对照研究。血清免疫球蛋白水平升高的患者库恩斯反应呈阳性，神经组织抗原发出明亮荧光。在库恩斯反应中，不同患者血清的反应也存在质的差异。这些数据表明，免疫球蛋白F(ab)2片段以及Fc片段都参与了免疫荧光反应，这就要求在解释该方法的结果时要谨慎。
