Lehn-Jensen H, Greve T
Nord Vet Med. 1981 Dec;33(12):523-9.
Blastocysts can survive direct transfer into liquid nitrogen after programmed freezing in 1.4 M glycerol to -29 degrees C when thawed by direct transfer into water at 37 degrees C. This observation facilitates the use of deep freezing of blastocysts in embryo transfer procedures. This makes the requirement for a special apparatus for the freezing procedure less demanding.
在1.4M甘油中程序冷冻至-29℃后的囊胚，经直接转移至37℃的水中解冻后，可直接转移至液氮中存活。这一观察结果便于在胚胎移植程序中对囊胚进行深度冷冻。这使得对冷冻程序专用设备的要求降低。
