Tosca A, Hatzis J, Haidas A, Varelzidis A
Dermatologica. 1981;163(5):401-7. doi: 10.1159/000250192.
The present study deals with the evaluation of some substrates for the detection of nuclear antibodies by the indirect immunofluorescence method. We have examined white male mouse liver imprint, white female pregnant mouse liver imprint, compound substrate consisting of male mouse liver, kidney and gastric wall sections, skin sections from psoriatic patients, skin sections from squamous cell carcinomata, male rabbit spleen imprint, skin sections from healthy adults people, skin sections from basal cell epitheliomata, skin sections from patients with lichen planus, male mouse spleen imprint and male guinea pig spleen imprint. To evaluate the substrates, we have employed 4 selected sera from patients with collagen disease. It was observed that some types of antinuclear antibodies demonstrate greater affinity for certain substrates and the fluorescent nuclear pattern might change on serial dilutions. From all the substrates studied, the white male mouse liver imprint and the male rabbit spleen imprint were considered as the most efficient for routine purpose as they are both sensitive and easily obtainable.
本研究涉及通过间接免疫荧光法评估用于检测核抗体的一些底物。我们检查了白色雄性小鼠肝脏印片、白色雌性妊娠小鼠肝脏印片、由雄性小鼠肝脏、肾脏和胃壁切片组成的复合底物、银屑病患者的皮肤切片、鳞状细胞癌的皮肤切片、雄性兔脾脏印片、健康成年人的皮肤切片、基底细胞上皮瘤的皮肤切片、扁平苔藓患者的皮肤切片、雄性小鼠脾脏印片和雄性豚鼠脾脏印片。为了评估这些底物，我们使用了4份选自胶原病患者的血清。观察到某些类型的抗核抗体对某些底物表现出更大的亲和力，并且荧光核模式可能在系列稀释时发生变化。在所有研究的底物中，白色雄性小鼠肝脏印片和雄性兔脾脏印片被认为是最适合常规用途的，因为它们既敏感又易于获得。
