Pape B E
Ther Drug Monit. 1981;3(4):357-63. doi: 10.1097/00007691-198104000-00006.
Quinidine, an antiarrhythmic drug, was quantitated in serum by a commercially supplied enzyme immunoassay procedure (EMIT). Replicate analyses of serum controls resulted in a within-assay coefficient of variation of less than or equal to 10% and a between-assay coefficient of variation of less than or equal to 7%. Patient quinidine results by enzyme immunoassay were compared to those obtained by a non-selective (protein precipitation) and a selective (solvent back-extraction) fluorometric method. Assay selectivity for quinidine versus other drugs and versus the 3-hydroxy metabolite of quinidine was determined. Clinical evaluation of the results indicates the enzyme immunoassay technique to be sensitive for quinidine and more selective than commonly used fluorometric methods relying on back-extraction or protein precipitation.
奎尼丁是一种抗心律失常药物，采用市售酶免疫分析程序（EMIT）对血清中的奎尼丁进行定量分析。对血清对照品进行重复分析，批内变异系数小于或等于10%，批间变异系数小于或等于7%。将酶免疫分析法测定的患者奎尼丁结果与通过非选择性（蛋白质沉淀）和选择性（溶剂反萃取）荧光法获得的结果进行比较。测定了该分析方法对奎尼丁与其他药物以及与奎尼丁3-羟基代谢物的选择性。结果的临床评估表明，酶免疫分析技术对奎尼丁敏感，并且比常用的依赖反萃取或蛋白质沉淀的荧光法更具选择性。
