[Importance of insulin- and thiamine-dependent processes in regulating lipid metabolism in the rat liver].

Lipid metabolism in liver tissue, adipocyte sizes and dynamics of body mass alteration were studied after administration of insulin, hydroxythiamine both and their mixtures into rats. In presence of insulin content of triglycerides as well as the rate of 6-3H-glucose incorporation into total lipids, fatty acids, phospholipids and triglycerides was increased in liver tissue. Administration of hydroxythiamine both into intact animals and into the rats treated with insulin led to decrease in content of fatty acids in liver tissue, triglycerides in liver tissue and blood serum as well as to lowering in the rate of 6-3H-glucose incorporation into fatty acids and triglycerides of liver tissue. Insulin activated and hydroxythiamine inhibited the liver tissue acetyl-CoA-carboxylase. Among other factors inhibition of lipogenesis appears to be the most important for decrease in the size of adipocytes and in body mass of the animals treated with hydroxythiamine.