Pedersen O, Hjøllund E, Lindskov H O, Beck-Nielsen H, Jensen J
Am J Physiol. 1982 Feb;242(2):E127-36. doi: 10.1152/ajpendo.1982.242.2.E127.
We have characterized the 24-h changes of insulin receptors on erythrocytes from patients with insulin-dependent diabetes of long duration. These diabetics were studied both in usual and poor metabolic control. Moreover, we have examined daytime changes of insulin receptors on monocytes from newly discovered diabetics. In both erythrocyte and monocyte studies, diabetics were compared to healthy controls. At insulin tracer concentration, insulin receptor binding to erythrocytes from diabetics in usual control and normal volunteers underwent a statistically significant diurnal variation with high binding values in the early morning, low daytime values with a nadir in the late afternoon, and a peak around midnight. Even diabetics in poor metabolic control due to insulin deprivation had preserved a similar 24-h rhythm of erythrocyte insulin receptors. Insulin receptor binding to monocytes at insulin tracer concentration declined significantly during the day both in newly discovered diabetics and in healthy controls. The mechanisms responsible for the acute phase changes of insulin-receptor binding are unknown, but the receptor changes seem related to the fed state. Moreover, analysis of the temporal interrelationship between erythrocyte insulin binding and plasma insulin concentration in diabetics during the 24-h period suggests that in these patients insulin may be one of the factors determining the rapid insulin receptor regulation.
我们已对病程较长的胰岛素依赖型糖尿病患者红细胞上胰岛素受体的24小时变化进行了特征描述。这些糖尿病患者在代谢控制正常和较差的情况下均接受了研究。此外，我们还检测了新发现糖尿病患者单核细胞上胰岛素受体的日间变化。在红细胞和单核细胞研究中，均将糖尿病患者与健康对照进行了比较。在胰岛素示踪剂浓度下，代谢控制正常的糖尿病患者及正常志愿者红细胞上的胰岛素受体结合呈现出具有统计学意义的昼夜变化，清晨结合值较高，白天较低，下午晚些时候降至最低点，午夜前后出现峰值。即使是因胰岛素缺乏导致代谢控制较差的糖尿病患者，其红细胞胰岛素受体也保持了类似的24小时节律。在胰岛素示踪剂浓度下，新发现糖尿病患者及健康对照的单核细胞上胰岛素受体结合在白天均显著下降。胰岛素受体结合急性期变化的机制尚不清楚，但受体变化似乎与进食状态有关。此外，对糖尿病患者24小时期间红细胞胰岛素结合与血浆胰岛素浓度之间时间相关性的分析表明，在这些患者中，胰岛素可能是决定胰岛素受体快速调节的因素之一。
