Dubuc P U, Leshin L S, Willis P L
Am J Physiol. 1982 Mar;242(3):R220-6. doi: 10.1152/ajpregu.1982.242.3.R220.
The effect of ventromedial (VMN) or basolateral (LHA) hypothalamic electrical stimulation on glucose and hormone production was examined in conscious and anesthetized male rats. Barbiturate treatment alone led to large increases of corticosterone and smaller but significant increases of glucose, insulin, and glucagon. When hypothalamic stimulation was combined with anesthesia, interactions between the hypothalamic sites and the awake-anesthetized conditions were observed. This was most evident with LHA placements, as awake rats exhibited mild hypoglycemia and hypoglucagonemia in response to low-level (approximately 10 microA rms) current, but no effects on these values were seen with VMN electrodes. With barbiturate anesthesia, the same level of stimulation led to severe hyperglycemia in both VMN and LHA rats and a mild hyperglucagonemia in the LHA group. No consistent effects of stimulation on immunoreactive insulin or corticosterone were detected at either hypothalamic site or in any anesthesia condition. The present results point out the important role anesthetics may play in studies defining functional aspects of the hypothalamus. Furthermore, our inability to elicit consistent autonomic responses with electrical stimulation, when compared with the effectiveness of both metabolic agents and pharmacologic stimuli, suggests that the sites integrating autonomic function may be diffusely distributed over the basal diencephalon.
在清醒和麻醉的雄性大鼠中，研究了下丘脑腹内侧核（VMN）或基底外侧核（LHA）电刺激对葡萄糖和激素分泌的影响。单独使用巴比妥类药物治疗导致皮质酮大幅增加，葡萄糖、胰岛素和胰高血糖素虽增加幅度较小但显著。当下丘脑刺激与麻醉相结合时，观察到下丘脑部位与清醒 - 麻醉状态之间的相互作用。这在LHA植入时最为明显，清醒大鼠对低水平（约10微安均方根）电流表现出轻度低血糖和低胰高血糖素血症，但VMN电极刺激对这些值没有影响。使用巴比妥类麻醉时，相同水平的刺激导致VMN和LHA大鼠均出现严重高血糖，LHA组出现轻度高胰高血糖素血症。在下丘脑的任何部位或任何麻醉条件下，均未检测到刺激对免疫反应性胰岛素或皮质酮有一致的影响。目前的结果指出了麻醉剂在确定下丘脑功能方面的研究中可能发挥的重要作用。此外，与代谢剂和药理刺激的有效性相比，我们无法通过电刺激引发一致的自主反应，这表明整合自主功能的部位可能广泛分布于基底间脑。
