Antheunisse J, de Bruin-Tol J W, van der Pol-van Soest M E
Antonie Van Leeuwenhoek. 1981;47(6):539-45. doi: 10.1007/BF00443240.
Bacteria, yeasts and fungi suspended in a dextran solution were added to ampoules containing strips of filter paper which were dried without vacuum conditions. The ampoules were sealed and stored in the dark at room temperature. Viability counts were made of the original suspension immediately after drying and after storage periods of 3-48 months. Although bacterial cultures of many general did not show much resistance against dry conditions, bacteria of 13 other general had survived well or moderately after 4 years of storage. Most of the dried yeast cultures had survived after this period. Of the 16 fungal genera tested, species of 6 genera exhibited growth after 4 years. Results of this study were compared with those of two other preservation methods by which the same microorganisms were used.
将悬浮在葡聚糖溶液中的细菌、酵母和真菌添加到装有滤纸条的安瓿中,滤纸条在无真空条件下干燥。安瓿密封后在室温黑暗环境中储存。在干燥后以及储存3至48个月后,对原始悬浮液进行活力计数。尽管许多属的细菌培养物对干燥条件没有很强的抵抗力,但其他13个属的细菌在储存4年后存活良好或中等。这段时间过后,大多数干燥的酵母培养物都存活了下来。在测试的16个真菌属中,6个属的物种在4年后仍有生长。本研究结果与使用相同微生物的其他两种保存方法的结果进行了比较。