Antheunisse J, Arkesteijn-Dijksman L
Antonie Van Leeuwenhoek. 1979;45(2):177-84. doi: 10.1007/BF00418582.
The survival rate of cells of the genera Arthrobacter, Pseudomonas, Mycobacterium, Escherichia, Micrococcus and Saccharomyces when counted immediately after fast or slow drying (20 minutes and 24 hours, respectively) was rather similar. However, after prolonged periods of dry storage, the number of viable cells after slow drying was much higher as compared with the rapidly dried cells. Investigations with Escherichia coli demonstrated this phenomenon only when more than about 8 mg of water per 10(8) cells was available on a filter paper disc. In order to obtain optimum resistance to water loss the dessication period of 0.025 ml of suspension of E. coli must be longer than 13 hours.
节杆菌属、假单胞菌属、分枝杆菌属、埃希氏菌属、微球菌属和酿酒酵母属的细胞在快速或慢速干燥后立即计数时(分别为20分钟和24小时),存活率相当相似。然而,经过长时间的干燥储存后,与快速干燥的细胞相比,慢速干燥后的活细胞数量要高得多。对大肠杆菌的研究表明,只有当滤纸片上每10⁸个细胞有超过约8毫克水时,才会出现这种现象。为了获得最佳的抗失水能力,0.025毫升大肠杆菌悬浮液的干燥时间必须超过13小时。