文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Eagles P A, Gilbert D S, Maggs A

Biochem J. 1981 Oct 1;199(1):101-11. doi: 10.1042/bj1990101.

When axoplasm is incubated with [32P]Pi the main phosphorylated components are the neurofilament polypeptides. 2. Activation with Ca2+ of the proteinase present in axoplasm causes degradation of these neurofilaments and the peptides produced by this reaction have been analysed by fingerprinting. 3. Fingerprinting shows that initially the Ca2+-activated proteinase cleaves the neurofilament polypeptides at three major sites producing polypeptides with mol.wts. 70,000, 50,000 and 47,000. 4. These polypeptides sediment with filaments, originate from the tail-region of the molecule and contain a little radioactive label. 5. As these polypeptides are produced, other polypeptides that come from the head-region of the molecule are liberated as soluble products that contain the bulk of the radioactivity. 6. Fingerprinting therefore shows that at least two regions on the molecule are phosphorylated and that the major one is located towards the head-end of the polypeptides.

当轴浆与[32P]磷酸孵育时，主要的磷酸化成分是神经丝多肽。2. 轴浆中存在的蛋白酶经Ca2+激活后会导致这些神经丝降解，并且已通过指纹图谱分析了该反应产生的肽。3. 指纹图谱显示，最初Ca2+激活的蛋白酶在三个主要位点切割神经丝多肽，产生分子量为70,000、50,000和47,000的多肽。4. 这些多肽与细丝一起沉降，起源于分子的尾部区域，并含有少量放射性标记。5. 随着这些多肽的产生，来自分子头部区域的其他多肽作为含有大部分放射性的可溶性产物被释放出来。6. 因此，指纹图谱表明分子上至少有两个区域被磷酸化，且主要区域位于多肽的头部末端。

Eagles P A, Gilbert D S, Maggs A

Biochem J. 1981 Oct 1;199(1):101-11. doi: 10.1042/bj1990101.

When axoplasm is incubated with [32P]Pi the main phosphorylated components are the neurofilament polypeptides. 2. Activation with Ca2+ of the proteinase present in axoplasm causes degradation of these neurofilaments and the peptides produced by this reaction have been analysed by fingerprinting. 3. Fingerprinting shows that initially the Ca2+-activated proteinase cleaves the neurofilament polypeptides at three major sites producing polypeptides with mol.wts. 70,000, 50,000 and 47,000. 4. These polypeptides sediment with filaments, originate from the tail-region of the molecule and contain a little radioactive label. 5. As these polypeptides are produced, other polypeptides that come from the head-region of the molecule are liberated as soluble products that contain the bulk of the radioactivity. 6. Fingerprinting therefore shows that at least two regions on the molecule are phosphorylated and that the major one is located towards the head-end of the polypeptides.

当轴浆与[32P]磷酸孵育时，主要的磷酸化成分是神经丝多肽。2. 轴浆中存在的蛋白酶经Ca2+激活后会导致这些神经丝降解，并且已通过指纹图谱分析了该反应产生的肽。3. 指纹图谱显示，最初Ca2+激活的蛋白酶在三个主要位点切割神经丝多肽，产生分子量为70,000、50,000和47,000的多肽。4. 这些多肽与细丝一起沉降，起源于分子的尾部区域，并含有少量放射性标记。5. 随着这些多肽的产生，来自分子头部区域的其他多肽作为含有大部分放射性的可溶性产物被释放出来。6. 因此，指纹图谱表明分子上至少有两个区域被磷酸化，且主要区域位于多肽的头部末端。