zur Nieden U, Neumann D, Manteuffel R, Weber E
Eur J Cell Biol. 1982 Feb;26(2):228-33.
The main storage proteins legumin and vicilin were located by immuno-labeling with protein A-gold in formaldehyde fixed tissues of Vicia faba seeds at different developmental stages. Both proteins were found to be accumulated together in the same protein bodies. Besides these organelles only the rough endoplasmic reticulum (ER) is labeled in particular developmental stages of the cotyledons. A fraction of proteins accumulated in the vacuoles at earlier stages of development also represents storage proteins. Besides the formation of protein bodies by the ER a second route of origination from vacuoles is proposed for Vicia faba seeds at early developmental stages.
通过用蛋白A-金免疫标记，在不同发育阶段的蚕豆种子甲醛固定组织中定位了主要贮藏蛋白豆球蛋白和豌豆球蛋白。发现这两种蛋白质共同积累在相同的蛋白体中。除了这些细胞器外，仅在子叶的特定发育阶段粗面内质网（ER）被标记。在发育早期积累在液泡中的一部分蛋白质也代表贮藏蛋白。除了内质网形成蛋白体外，还提出了蚕豆种子在发育早期从液泡产生的第二条途径。
