Zentralinstitut für Genetik und Kulturpflanzenforschung, Akademie der Wissenschaften der DDR, Corrensstrasse 3, DDR-4325, Gatersleben, German Democratic Republic.
Planta. 1983 Apr;157(5):401-10. doi: 10.1007/BF00397197.
Storage proteins of the field bean (Vicia faba L., var. minor, cv. "Fribo") are synthesized and accumulated in the cotyledons during stage 2 of seed development. Deposition of protein reserves takes place in the protein bodies. The generation of protein bodies was investigated electronmicroscopically using ultra-thin sections as well as the freeze-fracturing technique. During the initial period of storage protein formation, globulins are deposited in large vacuoles which later are transformed to give increasing numbers of small vacuoles with decreasing size. The vacuoles disappear early during the stage of storage protein formation and generate the first protein bodies. During the subsequent period of maximum storage protein formation, which takes place at the rough endoplasmic reticulum (rER), swollen ER strands appear which seem to be entirely filled with protein, and these generate ER-produced protein vacuoles (ERPVAC). The vesicles are transformed in a manner comparable to the vacuoles in the initial period of developmental stage 2 and thus generate the major quantity of protein bodies. Both processes seem to represent only two variants of an uniform mechanism of protein body generation.
菜豆（Vicia faba L.，var. minor，cv. "Fribo"）的贮藏蛋白在种子发育的第 2 阶段在子叶中合成和积累。蛋白质储备的沉积发生在蛋白质体中。使用超薄切片和冷冻断裂技术，电子显微镜研究了蛋白质体的生成。在贮藏蛋白形成的初始阶段，球蛋白沉积在大液泡中，这些液泡后来逐渐转化为越来越小的小液泡。在贮藏蛋白形成的早期阶段，液泡消失并产生第一批蛋白质体。在随后的最大贮藏蛋白形成阶段，发生在粗面内质网（rER）上，出现肿胀的内质网链，这些内质网链似乎完全充满了蛋白质，这些生成内质网产生的蛋白质空泡（ERPVAC）。这些囊泡的转化方式与发育阶段 2 初始阶段的液泡类似，从而产生大量的蛋白质体。这两个过程似乎只是蛋白质体生成的统一机制的两种变体。
