鼠伤寒沙门氏菌温度敏感致死突变体的分离与鉴定，该突变体在3-脱氧-D-甘露糖辛酮酸-8-磷酸合成中存在条件性缺陷。
Isolation and characterization of a temperature-sensitive lethal mutant of Salmonella typhimurium that is conditionally defective in 3-deoxy-D-manno-octulosonate-8-phosphate synthesis.
作者信息
Rick P D, Young D A
出版信息
J Bacteriol. 1982 May;150(2):447-55. doi: 10.1128/jb.150.2.447-455.1982.
Abstract
A new mutant of Salmonella typhimurium was isolated which possesses a temperature-sensitive defect in the synthesis of 3-deoxy-D-manno-octulosonic acid. The defect in 3-deoxy-D-manno-octulosonic acid synthesis is due to a temperature-sensitive 3-deoxy-D-manno-octulosonate-8-phosphate synthetase, and the mutant accumulates an incomplete lipid A under nonpermissive conditions. Evidence is presented which indicates that the incomplete lipid A molecule is most likely identical in structure to the lipid A precursor synthesized by previously characterized mutants conditionally defective in 3-deoxy-D-manno-octulosonic acid synthesis. However, unlike related mutants which undergo growth stasis under nonpermissive conditions, the accumulation of lipid A precursor in the new mutant results in cell death at elevated temperatures.
摘要
分离出一种鼠伤寒沙门氏菌的新突变体，它在3-脱氧-D-甘露糖辛酮酸合成中存在温度敏感缺陷。3-脱氧-D-甘露糖辛酮酸合成缺陷是由于一种温度敏感的3-脱氧-D-甘露糖辛酮酸-8-磷酸合成酶，并且该突变体在非允许条件下积累不完全脂多糖A。提供的证据表明，不完全脂多糖A分子的结构很可能与先前鉴定的在3-脱氧-D-甘露糖辛酮酸合成中有条件缺陷的突变体合成的脂多糖A前体相同。然而，与在非允许条件下经历生长停滞的相关突变体不同，新突变体中脂多糖A前体的积累导致在高温下细胞死亡。
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Isolation and characterization of a temperature-sensitive lethal mutant of Salmonella typhimurium that is conditionally defective in 3-deoxy-D-manno-octulosonate-8-phosphate synthesis.鼠伤寒沙门氏菌温度敏感致死突变体的分离与鉴定，该突变体在3-脱氧-D-甘露糖辛酮酸-8-磷酸合成中存在条件性缺陷。
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