Fox B, Shousha S, James K R, Miller G C
J Clin Pathol. 1982 Feb;35(2):144-50. doi: 10.1136/jcp.35.2.144.
An unlabelled antibody peroxidase-antiperoxidase method for the detection of IgG, IgM, complement (C3 and Clq), fibrinogen and albumin was applied to routinely processed paraffin sections of lung from 27 cases. The results in 11 cases were compared with those obtained by immunofluorescence using frozen sections. Tissue was obtained from surgical specimens of cases with interstitial pneumonia comprising 10 of the usual type (UIP) and three of the desquamative type (DIP). Tissue was also obtained from the specimens of cases with sarcoidosis (two cases) and granulomatous inflammation of unknown cause (one case). There were 11 control cases, nine with primary carcinoma of the lung and two with metastatic tumours of the lung. Immunoglobulins of various types and complement were seen in diseased lung tissue. Although most of these deposits were probably due to a non-immunological mechanism there was evidence of the possible implication of immune complexes in three cases of UIP and in the interstitial pneumonia present in the two cases of sarcoidosis. The immunoperoxidase technique is a more sensitive method than immunofluorescence and has the additional advantage of the easy identification of the precise sites of the various deposits.
一种用于检测IgG、IgM、补体（C3和Clq）、纤维蛋白原和白蛋白的未标记抗体过氧化物酶-抗过氧化物酶方法应用于27例经常规处理的肺石蜡切片。将11例的结果与使用冷冻切片通过免疫荧光获得的结果进行比较。组织取自间质性肺炎病例的手术标本，其中包括10例普通型（UIP）和3例脱屑型（DIP）。组织还取自结节病病例（2例）和病因不明的肉芽肿性炎症病例（1例）的标本。有11例对照病例，9例原发性肺癌和2例肺转移瘤。在患病肺组织中可见各种类型的免疫球蛋白和补体。尽管这些沉积物中的大多数可能是由于非免疫机制，但有证据表明在3例UIP和2例结节病中的间质性肺炎中免疫复合物可能起作用。免疫过氧化物酶技术是一种比免疫荧光更敏感的方法，并且具有易于识别各种沉积物精确部位的额外优势。
