Brode E
J Clin Chem Clin Biochem. 1982 Jan;20(1):39-47.
A high performance liquid chromatography method based on the principle of direct isotope dilution is described for the determination of propafenone in plasma. With a sample volume of 2 ml and quantification by peak height measurements the lower limit of detection is about 2.5 micrograms/l, and the lower limit of determination about 10 micrograms/l. Values up to 250 micrograms/l are subject to a mean weighted relative error of 3.5%. At concentrations above 20 micrograms/l this method shows the same accuracy and reproducibility as the analytical procedure employing internal standardization with analogues. The method is made independent of possible storage losses by internal standardization of each sample immediately after withdrawal.
描述了一种基于直接同位素稀释原理的高效液相色谱法，用于测定血浆中的普罗帕酮。进样量为2 ml，通过峰高测量进行定量，检测下限约为2.5微克/升，定量下限约为10微克/升。高达250微克/升的值的平均加权相对误差为3.5%。在浓度高于20微克/升时，该方法与采用类似物内标法的分析程序具有相同的准确度和重现性。通过在每个样品取出后立即进行内标，该方法不受可能的储存损失的影响。
