穿孔电子显微镜载片上细胞核的裂解
Lysis of cell nuclei on perforated electron microscopic supports.
作者信息
Erenpreisa J
出版信息
Microsc Acta. 1982 Mar;85(4):369-80.
PMID:7040913
Abstract
The technique of gentle nuclear lysis for electron microscopic observation is suggested, which comprises adsorption of isolated nuclei on a positively charged pricked supporting film and sequential short-timed installation of the support with its back onto the filter paper moistened by lysing solution. The advantage of the method is slow, gradient-like, and to some extent regulated treatment allowing to visualize nuclear structures in their integrity.
摘要
建议采用温和核裂解技术用于电子显微镜观察，该技术包括将分离的细胞核吸附在带正电荷的穿孔支撑膜上，然后将支撑膜背面依次短时间放置在用裂解液浸湿的滤纸上。该方法的优点是处理过程缓慢、呈梯度状且在一定程度上可调节，能够完整地观察到核结构。
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