Schmalzl F, Wiedermann C J, Braunsteiner H
Acta Med Austriaca. 1982;9(1):29-34.
The acid phosphatase activity (acP) of developing skin window macrophages topically treated with 6-methyl-prednisolone (6-MP) has been evaluated by a quantitative-cytochemical technique (SMP 05/PDP 12-20). The following conclusions can be drawn: 1. The degranulation of migrated monocytes can be inhibited by local application of 0.1 to 10.0 micrograms of 6-MP per skin window lesion. 2. The increase of acP-activity associated with the physiologic transformation of monocytes into macrophages and the concomitant activation of the lysosomal system was clearly inhibited by 0.05 to 10.0 micrograms per skin window lesion. 3. 6-MP alters the morphology of the developing macrophages and alters the intracellular distribution of acP. 4. It can be concluded that 6-MP inhibits the development of monocytogenic macrophages. This effect is observed with amounts of 6-MP which correspond to those obtained by systemic application of 6-MP in usual dosages. The antiphlogistic effect of 6-MP probably is due to a considerable proportion by the direct influence on the inflammatory cells.
采用定量细胞化学技术（SMP 05/PDP 12 - 20）评估了用6 - 甲基泼尼松龙（6 - MP）局部处理的皮肤窗口巨噬细胞的酸性磷酸酶活性（acP）。可得出以下结论：1. 每皮肤窗口损伤局部应用0.1至10.0微克的6 - MP可抑制迁移单核细胞的脱颗粒。2. 每皮肤窗口损伤0.05至10.0微克的6 - MP可明显抑制与单核细胞向巨噬细胞的生理转化以及溶酶体系统的伴随激活相关的acP活性增加。3. 6 - MP改变发育中巨噬细胞的形态并改变acP的细胞内分布。4. 可以得出结论，6 - MP抑制单核细胞源性巨噬细胞的发育。在与通常剂量全身应用6 - MP所获得的量相对应的6 - MP量下观察到这种效应。6 - MP的抗炎作用可能相当一部分归因于对炎症细胞的直接影响。
