Hsueh W, Lamb R, Gonzalez-Crussi F
Biochim Biophys Acta. 1982 Mar 12;710(3):406-14. doi: 10.1016/0005-2760(82)90124-2.
Alveolar macrophages from normal and BCG-infected rabbits were labelled with [14C]arachidonate and its metabolites were measured. It was found that production of both prostaglandins and 12-hydroxyeicosatetraenoic acid was diminished markedly in BCG-primed macrophages. This decrease was due partly to a depression in cyclooxygenase and lipoxygenase activity, but mainly to a decrease in arachidonic acid release, probably due to a suppression in phospholipase A2 activity. This is indicated by a consistent depression of this enzyme activity in BCG macrophage homogenates at a wide pH range, suggesting that both lysosomal an non-lysosomal phospholipases are suppressed in BCG macrophages. However, intracellular lysosomal acid phosphatase and its release were increased markedly in BCG-primed macrophages. Our previous studies have shown a close relationship between lysosomal acid hydrolase release and production of arachidonate-prostaglandins in normal macrophages. The present study shows that in activated macrophages primed by BCG, different mechanisms are operative in the control of synthesis and release of lysosomal acid hydrolases and of the phospholipase for prostaglandin production.
用[14C]花生四烯酸盐标记正常兔和卡介苗感染兔的肺泡巨噬细胞，并测定其代谢产物。结果发现，卡介苗致敏的巨噬细胞中前列腺素和12-羟基二十碳四烯酸的生成均显著减少。这种减少部分是由于环氧化酶和脂氧化酶活性降低，但主要是由于花生四烯酸释放减少，这可能是由于磷脂酶A2活性受到抑制。卡介苗巨噬细胞匀浆在很宽的pH范围内该酶活性持续降低，表明溶酶体和非溶酶体磷脂酶在卡介苗巨噬细胞中均受到抑制。然而，卡介苗致敏的巨噬细胞中细胞内溶酶体酸性磷酸酶及其释放显著增加。我们之前的研究表明，正常巨噬细胞中溶酶体酸性水解酶释放与花生四烯酸-前列腺素生成之间存在密切关系。本研究表明，在卡介苗致敏的活化巨噬细胞中，溶酶体酸性水解酶和前列腺素生成所需磷脂酶的合成与释放控制存在不同机制。
