肺泡巨噬细胞中磷脂酶A对二棕榈酰卵磷脂的去酰基作用。

Deacylation of dipalmitoyllecithin by phospholipases A in alveolar macrophages.

作者信息

Rao R H, Waite M, Myrvik Q N

出版信息

Exp Lung Res. 1981 Mar;2(1):9-15. doi: 10.3109/01902148109052298.

DOI:10.3109/01902148109052298

Abstract

Optimal conditions in vitro were established for the assay of phospholipases A of Bacillus Calmette Guerin-induced alveolar macrophages that deacylated dipalmitoyllecithin in the alkaline pH range. Sodium deoxycholate and Ca++ were found to stimulate both phospholipases A1 and A2, whereas sodium dodecylsulfate strongly inhibited both enzyme activities. Other detergents like Triton X-100, Triton WR-1339, and Tween-20 activated phospholipase A1, whereas they had no effect on or slightly inhibited the phospholipase A2 enzyme. These phospholipases preferentially removed palmitic acid regardless of the position of palmitic acid on the phospholipid molecule and were more active on phosphatidylcholines than phosphatidylethanolamines. This suggests a role for macrophage phospholipases A in the clearance of saturated lecithins in the pulmonary surfactant complex.

摘要

建立了体外最佳条件，用于检测卡介苗诱导的肺泡巨噬细胞的磷脂酶A，该酶在碱性pH范围内使二棕榈酰卵磷脂脱酰基。发现脱氧胆酸钠和Ca++能刺激磷脂酶A1和A2，而十二烷基硫酸钠强烈抑制这两种酶的活性。其他洗涤剂如 Triton X-100、Triton WR-1339 和吐温-20 激活磷脂酶A1，而它们对磷脂酶A2没有影响或略有抑制。这些磷脂酶优先去除棕榈酸，而不管棕榈酸在磷脂分子上的位置如何，并且对磷脂酰胆碱的活性比对磷脂酰乙醇胺的活性更高。这表明巨噬细胞磷脂酶A在清除肺表面活性物质复合物中的饱和卵磷脂中起作用。