DeRuyter H, Burman K D, Wartofsky L, Taylor S I
Endocrinology. 1982 Jun;110(6):1922-5. doi: 10.1210/endo-110-6-1922.
Although many studies have investigated the complex interrelationships between thyroid hormone levels and insulin-glucose secretion and action, there is at present a lack of information concerning the effects of various thyroid hormone levels on insulin receptors. In the present study, rat liver membranes were prepared from control, hyperthyroid [10 micrograms L-T4 (T4)/100 g BW for 14 days], and hypothyroid rats and the binding characteristics of [125I]iodoinsulin to these membranes were studied. In addition, serum T3, T4, glucose, and insulin levels were measured. The mean (+/- SD) serum T4 and T3 levels were higher (P less than 0.05) in the T4-injected rats than in the hypothyroid rats (T4, 9.1 +/- 0.6 vs. 1.7 +/- 0.4 microgram/dl; T3, 144 +/- 18 vs. 30 +/- 16 ng/dl). When compared to controls, glucose levels were higher in the hypothyroid rats (116 +/- 11 vs. 141 +/- 14 mg/dl; P less than 0.05) and were statistically unaltered in thyrotoxicosis. Plasma insulin levels were increased in hypothyroidism (6.5 +/- 0.8 vs. 10.8 +/- 2.9 microU/ml; P less than 0.05) and decreased in thyrotoxicosis (7.5 +/- 0.5 vs. 28.3 +/- 15 microU/ml; P less than 0.05). Despite these alterations, membrane binding and the derived Scatchard plots were not significantly different in the hyperthyroid or hypothyroid groups. In summary, the present studies indicate that plasma membrane insulin receptor number and affinity are unaltered regardless of the thyroid state of the rat.
尽管许多研究已经探讨了甲状腺激素水平与胰岛素 - 葡萄糖分泌及作用之间的复杂相互关系，但目前关于不同甲状腺激素水平对胰岛素受体的影响仍缺乏相关信息。在本研究中，从对照大鼠、甲状腺功能亢进大鼠（10微克L - T4（T4）/100克体重，持续14天）和甲状腺功能减退大鼠制备大鼠肝细胞膜，并研究[125I]碘胰岛素与这些细胞膜的结合特性。此外，还测量了血清T3、T4、葡萄糖和胰岛素水平。注射T4的大鼠血清T4和T3的平均（±标准差）水平高于甲状腺功能减退大鼠（P < 0.05）（T4，9.1 ± 0.6对1.7 ± 0.4微克/分升；T3，144 ± 18对30 ± 16纳克/分升）。与对照组相比，甲状腺功能减退大鼠的葡萄糖水平较高（116 ± 11对141 ± 14毫克/分升；P < 0.05），而甲状腺毒症时葡萄糖水平无统计学改变。甲状腺功能减退时血浆胰岛素水平升高（6.5 ± 0.8对10.8 ± 2.9微单位/毫升；P < 0.05），甲状腺毒症时降低（7.5 ± 0.5对28.3 ± 15微单位/毫升；P < 0.05）。尽管有这些改变，但甲状腺功能亢进或减退组的膜结合及由此得到的Scatchard图并无显著差异。总之,本研究表明，无论大鼠的甲状腺状态如何，质膜胰岛素受体的数量和亲和力均未改变。
