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大鼠下丘脑切片中的室旁神经元:荧光黄注射与免疫细胞化学鉴定

Paraventricular neurones in the rat hypothalamic slice: Lucifer Yellow injection and immunocytochemical identification.

作者信息

Kayser B E, Mühlethaler M, Dreifuss J J

出版信息

Experientia. 1982 Mar 15;38(3):391-3. doi: 10.1007/BF01949412.

DOI:10.1007/BF01949412
PMID:7042380
Abstract

Intracellular records were obtained from paraventricular neurones injected with Lucifer Yellow in slices from the rat hypothalamus. Slices containing fluorescent neurones were then serially cut and alternating sections were stained immunocytochemically for vasopressin or oxytocin. Double-labelled cells were found which fluoresced and which had reacted with either vasopressin or oxytocin antiserum.

摘要

在大鼠下丘脑切片中,从注射了荧光黄的室旁神经元获取细胞内记录。然后对含有荧光神经元的切片进行连续切割,并对交替的切片进行抗血管加压素或抗催产素的免疫细胞化学染色。发现了双标记细胞,这些细胞发出荧光并且与血管加压素或催产素抗血清发生反应。

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1
Paraventricular neurones in the rat hypothalamic slice: Lucifer Yellow injection and immunocytochemical identification.大鼠下丘脑切片中的室旁神经元:荧光黄注射与免疫细胞化学鉴定
Experientia. 1982 Mar 15;38(3):391-3. doi: 10.1007/BF01949412.
2
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3
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Immunoreactivity to vasopressin- but not oxytocin-associated neurophysin antiserum in phasic neurons of rat hypothalamic paraventricular nucleus.大鼠下丘脑室旁核相位神经元中对血管加压素相关神经垂体素抗血清呈免疫反应,而对催产素相关神经垂体素抗血清无免疫反应。
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Colocalization of calbindin-D28k with vasopressin in hypothalamic cells of the rat: a double-labeling immunofluorescence study.大鼠下丘脑细胞中钙结合蛋白-D28k与血管加压素的共定位:一项双标记免疫荧光研究。
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本文引用的文献

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Temporal patterns of neural activity and their relation to the secretion of posterior pituitary hormones.神经活动的时间模式及其与垂体后叶激素分泌的关系。
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大鼠下丘脑室旁核的亚核:细胞构筑学、辣根过氧化物酶及免疫细胞化学分析
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Electrophysiological studies of paraventricular and supraoptic neurones recorded in vitro from slices of rat hypothalamus.对从大鼠下丘脑切片中体外记录的室旁核和视上核神经元的电生理研究。
J Physiol. 1980 May;302:347-62. doi: 10.1113/jphysiol.1980.sp013247.
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Intracellular recordings from the paraventricular nucleus in slices of rat hypothalamus.来自大鼠下丘脑切片室旁核的细胞内记录。
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Labeling of neurons within CNS explants by intracellular injection of lucifer yellow.通过向中枢神经系统外植体中的神经元内注射荧光黄进行标记。
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Hyperpolarization of hypothalamic parvocellular neurons by 17 beta-estradiol and their identification through intracellular staining with procion yellow.17β-雌二醇对下丘脑小细胞神经元的超极化作用及其通过普施安黄进行细胞内染色的鉴定
Exp Brain Res. 1980;40(4):440-7. doi: 10.1007/BF00236152.
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Morphine and opioid peptides reduce paraventricular neuronal activity: studies on the rat hypothalamic slice preparation.吗啡和阿片肽降低室旁核神经元活动:对大鼠下丘脑脑片标本的研究。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5527-31. doi: 10.1073/pnas.77.9.5527.
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Enkephalin-induced inhibition of hypothalmaic paraventricular neurons.
Brain Res. 1980 Sep 15;197(1):264-8. doi: 10.1016/0006-8993(80)90457-6.