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大鼠下丘脑切片中的室旁神经元:荧光黄注射与免疫细胞化学鉴定

Paraventricular neurones in the rat hypothalamic slice: Lucifer Yellow injection and immunocytochemical identification.

作者信息

Kayser B E, Mühlethaler M, Dreifuss J J

出版信息

Experientia. 1982 Mar 15;38(3):391-3. doi: 10.1007/BF01949412.

Abstract

Intracellular records were obtained from paraventricular neurones injected with Lucifer Yellow in slices from the rat hypothalamus. Slices containing fluorescent neurones were then serially cut and alternating sections were stained immunocytochemically for vasopressin or oxytocin. Double-labelled cells were found which fluoresced and which had reacted with either vasopressin or oxytocin antiserum.

摘要

在大鼠下丘脑切片中,从注射了荧光黄的室旁神经元获取细胞内记录。然后对含有荧光神经元的切片进行连续切割,并对交替的切片进行抗血管加压素或抗催产素的免疫细胞化学染色。发现了双标记细胞,这些细胞发出荧光并且与血管加压素或催产素抗血清发生反应。

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