Masuda A, Akiyama S, Kuwano M, Ikekawa N
J Antibiot (Tokyo). 1982 Feb;35(2):230-4. doi: 10.7164/antibiotics.35.230.
The antifungal effect of a polyene antibiotic amphotericin B (AMB) was almost completely abrogated by exogenous addition of 0.1 microgram/ml ergosterol in the medium. The cytocidal effect of AMB on Saccharomyces cerevisiae was synergistically enhanced when cultured for more than 4 hours with squalene, an obligatory intermediate molecule for sterol formation. However, we could not find significant increase in cellular level of ergosterol content in the yeast cells fed with squalene.
在培养基中外源添加0.1微克/毫升麦角固醇后，多烯抗生素两性霉素B（AMB）的抗真菌作用几乎完全被消除。当与角鲨烯（固醇形成的必需中间分子）一起培养超过4小时时，AMB对酿酒酵母的杀细胞作用会协同增强。然而，我们并未发现用角鲨烯喂养的酵母细胞中麦角固醇含量的细胞水平有显著增加。
