质粒原噬菌体P1的突变体与拷贝数增加：分离与鉴定
Mutants of plasmid prophage P1 and elevated copy number: isolation and characterization.
作者信息
Scott J R, Kropf M M, Padolsky L, Goodspeed J K, Davis R, Vapnek D
出版信息
J Bacteriol. 1982 Jun;150(3):1329-39. doi: 10.1128/jb.150.3.1329-1339.1982.
Abstract
A series of mutations of the P1 plasmid prophage that lead to increased copy number was isolated and analyzed. The copy number of the mutants was elevated at least five- to eightfold relative to wild-type P1, as determined by single-cell resistance to antibiotics, activity of enzymes, content of superhelical DNA, and reassociation kinetics. The copy number of two of the mutants was temperature dependent. Based on dominance tests, the mutants fell into two classes, cis specific and recessive. The latter class included a temperature-sensitive copy mutant. The existence of a class of recessive mutants suggests that the replication of the P1 plasmid is negatively regulated.
摘要
分离并分析了一系列导致P1质粒原噬菌体拷贝数增加的突变。通过单细胞对抗生素的抗性、酶活性、超螺旋DNA含量和重缔合动力学确定，突变体的拷贝数相对于野生型P1至少提高了五到八倍。其中两个突变体的拷贝数是温度依赖性的。基于显性测试，突变体分为两类，顺式特异性和隐性。后一类包括一个温度敏感型拷贝突变体。一类隐性突变体的存在表明P1质粒的复制受到负调控。
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