Lerch R A, Bergmann S R, Carlson E M, Saffitz J E, Sobel B E
J Nucl Med. 1982 Jun;23(6):496-500.
To determine whether lymphocytes labeled with indium-111 permit noninvasive assessment of antirejection therapy, we performed 40 allogeneic heterotopic cardiac transplants in rats. Antirejection therapy with azathioprine (30 mg/kg) and sodium salicylate (200 mg/kg) prolonged contractile function of the graft from 7.5 +/- 1.5 (s.d.) days in controls to 19.4 +/- 3.7 days in treated animals. Six to seven days after transplantation, autologous lymphocytes labeled with In-111 were injected intravenously in seven untreated and eight treated rats. Scintigraphy and organ counting were performed 24 hr after administration of labeled cells. At sacrifice all grafts in untreated rats exhibited contractile failure, whereas grafts in all treated rats were beating well. Transplants in untreated recipients exhibited marked accumulation of In-111 lymphocytes detectable scintigraphically, with ratios of 7.7 +/- 1.9 for the activity in the transplant over that in the native heart (HT/HO), as obtained by well counting. In contrast, accumulation was not scintigraphically detectable in transplants of treated rats, with HT/HO ratios of 2.6 +/- 1.8 (p less than 0.005). The results suggested that imaging with in-111-labeled lymphocytes will permit noninvasive assessment of antirejection therapy.
为了确定用铟 - 111标记的淋巴细胞是否能用于抗排斥治疗的无创评估，我们在大鼠身上进行了40例同种异体异位心脏移植。用硫唑嘌呤（30毫克/千克）和水杨酸钠（200毫克/千克）进行抗排斥治疗，使移植心脏的收缩功能从对照组的7.5±1.5（标准差）天延长至治疗组动物的19.4±3.7天。移植后6至7天，给7只未治疗和8只治疗的大鼠静脉注射用铟 - 111标记的自体淋巴细胞。在注射标记细胞24小时后进行闪烁扫描和器官计数。处死时，未治疗大鼠的所有移植心脏均出现收缩功能衰竭，而所有治疗大鼠的移植心脏跳动良好。未治疗受体的移植心脏通过井型计数器检测到铟 - 111淋巴细胞明显积聚，移植心脏与自身心脏的放射性活度比（HT/HO）为7.7±1.9。相比之下，治疗大鼠的移植心脏在闪烁扫描中未检测到积聚，HT/HO比值为2.6±1.8（p<0.005）。结果表明，用铟 - 111标记的淋巴细胞进行成像将允许对抗排斥治疗进行无创评估。
