Thorndyke M C
Regul Pept. 1982 Mar;3(3-4):281-8. doi: 10.1016/0167-0115(82)90133-1.
Antisera raised against the COOH-terminal sequence of mammalian CCK/gastrin were used to ascertain the distribution of CCK/gastrin-like immunoreactive cells in the cerebral ganglion of two ascidian protochordates. Styela clava and Ascidiella aspersa. the cell bodies were found to have a specific regional distribution in Ascidiella, but not in Styela. In addition to central immunoreactive nerve fibres, a number of peripherally located immunoreactive fibres was found. These observations support the idea that some centrally originating protochordate neuronal peptides may have a peripheral role and give weight to the hypothesis that many vertebrate brain-gut peptides had their origin in the neuronal elements of more primitive species.
用针对哺乳动物缩胆囊素/胃泌素COOH末端序列产生的抗血清，来确定两种海鞘原索动物（柄海鞘和粗糙海鞘）脑神经节中缩胆囊素/胃泌素样免疫反应性细胞的分布。结果发现，在粗糙海鞘中，细胞体具有特定的区域分布，而在柄海鞘中则没有。除了中枢免疫反应性神经纤维外，还发现了一些位于外周的免疫反应性纤维。这些观察结果支持了这样一种观点，即一些起源于中枢的原索动物神经肽可能具有外周作用，也支持了许多脊椎动物脑肠肽起源于更原始物种的神经元成分这一假说。
