Vigna S R, Morgan J L, Thomas T M
J Neurosci. 1984 May;4(5):1370-7. doi: 10.1523/JNEUROSCI.04-05-01370.1984.
Gastrin/cholecystokinin-like immunoreactivity (G/CCK-LI) was localized by immunocytochemistry in neurons in the central nervous system of Aplysia californica. Neuronal cell bodies were specifically immunostained in the buccal, cerebral, pedal, and abdominal ganglia but not in the pleural ganglia. Neural G/CCK-LI processes were observed in the neuropil of all ganglia and connectives and in some but not all of the peripheral nerves examined. Somata containing G/CCK-LI ranged from 15 to 200 micron in diameter. Ganglionic G/CCK-LI was most efficiently extracted in neutral or basic solutions and consisted mainly of a substance eluting in a volume corresponding to a peptide of between 8 and 17 amino acid residues on gel filtration. This G/CCK-LI diluted in parallel to mammalian gastrin in radioimmunoassays using two antisera differing in their specificities for the bioactive COOH-terminal region of mammalian G/CCK. We conclude that G/CCK-LI is distributed widely in the central and peripheral nervous systems of Aplysia californica and that this immunoreactivity probably represents a small peptide which is similar but not identical to mammalian gastrins and cholecystokinins at the functionally critical COOH terminus.
通过免疫细胞化学方法，在加州海兔中枢神经系统的神经元中定位了胃泌素/胆囊收缩素样免疫反应性物质（G/CCK-LI）。在口神经节、脑侧神经节、足神经节和腹神经节中，神经元细胞体出现特异性免疫染色，而在胸膜神经节中未出现。在所有神经节、神经连索的神经纤维网以及部分（而非全部）所检查的外周神经中观察到了神经G/CCK-LI的神经突。含有G/CCK-LI的细胞体直径在15至200微米之间。神经节中的G/CCK-LI在中性或碱性溶液中提取效率最高，在凝胶过滤中，其主要成分洗脱体积对应的物质相当于一个含有8至17个氨基酸残基的肽。在放射免疫分析中，使用两种对哺乳动物G/CCK生物活性COOH末端区域特异性不同的抗血清，这种G/CCK-LI与哺乳动物胃泌素呈平行稀释。我们得出结论，G/CCK-LI广泛分布于加州海兔的中枢和外周神经系统，并且这种免疫反应性可能代表一种小肽，在功能关键的COOH末端与哺乳动物胃泌素和胆囊收缩素相似但并不相同。
