Bajwa S S, Kirakossian H, Reddy K N, Markland F S
Toxicon. 1982;20(2):427-32. doi: 10.1016/0041-0101(82)90005-8.
Crude venom from B. gabonica contained weak fibrinogen clotting activity but no visible fibrinolytic activity, whereas venoms from A. p. piscivorus and A. c. contortrix exhibited fibrinolytic activity (by fibrin plate assay) but no thrombin-like activity. These snake venoms were fractionated on Sephadex G-100 with the following results. Thrombin-like activity in B. gabonica venom was eluted in a single protein peak with a molecular weight of 40,000. Agkistrodon p. piscivorus venom contained a single peak of fibrinolytic activity with a molecular weight of 34,000. Interestingly, venom from A. c. contortrix, which showed no thrombin-like activity in crude venom, contained both thrombin-like and fibrinolytic activities in fractions with molecular weights of 73,000 and 25,000 respectively. No plasminogen activation activity was observed in any of the crude venoms or venom fractions eluted from G-100. In view of the possible clinical potential of these enzymes as defibrinogenating or thrombolytic agents, it will be of great interest to further purify and characterize them.
加蓬咝蝰的粗毒含有较弱的纤维蛋白原凝血活性，但无明显的纤溶活性，而食鱼蝮和铜头蝮的毒液表现出纤溶活性（通过纤维蛋白平板试验），但无类凝血酶活性。这些蛇毒在葡聚糖G - 100上进行分级分离，结果如下。加蓬咝蝰毒液中的类凝血酶活性在一个分子量为40,000的单一蛋白峰中洗脱出来。食鱼蝮毒液含有一个分子量为34,000的单一纤溶活性峰。有趣的是，粗毒中无类凝血酶活性的铜头蝮毒液，在分子量分别为73,000和25,000的组分中同时含有类凝血酶和纤溶活性。在从G - 100洗脱的任何粗毒或毒液组分中均未观察到纤溶酶原激活活性。鉴于这些酶作为去纤维蛋白原或溶栓剂可能具有的临床潜力，进一步纯化和鉴定它们将具有极大的意义。
