Reddy N R, Pierson M D, Lechowich R V
Appl Environ Microbiol. 1982 Apr;43(4):835-9. doi: 10.1128/aem.43.4.835-839.1982.
A total of 75 compounds, including antioxidants, preservatives, gallic acid and p-hydroxybenzoic acid esters, hydroquinones, hydroxyquinolines, phenol derivatives, and related compounds, were screened for their antibotulinal activity in prereduced Thiotone-yeast extract-glucose broth. The most effective inhibitors of Clostridium botulinum growth and toxin production were long-chain esters of p-hydroxybenzoic acid and gallic acid, antioxidants, and butylphenol derivatives. The antioxidant nordihydroguaiaretic acid at 100 microgram/ml delayed the growth and toxin production for the entire incubation period (7 days). Other antioxidants, such as butylated hydroxytoluene, butylated hydroxyanisole, and tert-butylhydroquinone were also very effective (at 200 to 400 microgram/ml) for the inhibition of C. botulinum growth and toxin production. Toxin was detected, although no detectable growth was found by daily absorbance measurements, in the prereduced medium containing 50 to 400 microgram of 8-hydroxyquinoline, pentylphenol, tert-pentylphenol, 3,5-ditert-butylphenol, 3,5-ditert-butylcatechol, (2-hydroxydiphenyl)methane, or (4-hydroxydiphenyl)methane per ml.
总共75种化合物，包括抗氧化剂、防腐剂、没食子酸和对羟基苯甲酸酯、对苯二酚、羟基喹啉、酚类衍生物及相关化合物，在预还原的硫代tone -酵母提取物 -葡萄糖肉汤中进行了抗肉毒杆菌活性筛选。对肉毒梭菌生长和毒素产生最有效的抑制剂是对羟基苯甲酸和没食子酸的长链酯、抗氧化剂和丁基酚衍生物。100微克/毫升的抗氧化剂去甲二氢愈创木酸在整个培养期（7天）内延迟了生长和毒素产生。其他抗氧化剂，如丁基化羟基甲苯、丁基化羟基茴香醚和叔丁基对苯二酚（200至400微克/毫升）对抑制肉毒梭菌生长和毒素产生也非常有效。在每毫升含有50至400微克8 -羟基喹啉、戊基酚、叔戊基酚、3,5 -二叔丁基酚、3,5 -二叔丁基儿茶酚、（2 -羟基二苯基）甲烷或（4 -羟基二苯基）甲烷的预还原培养基中，尽管通过每日吸光度测量未发现可检测到的生长，但检测到了毒素。
